Nucleoside diphosphate kinase/Nm23 and Epstein–Barr virus
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چکیده
منابع مشابه
Hydroxyurea and Escherichia coli nucleoside diphosphate reductase.
M). The cultures were incubated at 37° for 40 mm (“Pretreatment―), whereupon the bacteria were washed and resuspended in fresh Medium HA. Portions from each culture were distributed into flasks containing premeasured amounts of the various drugs (“Additions―). After receiving cytidine-3H (2 @ig/ml; 0.13 i@Ci/ml),the cultures were incubated with aeration at 37°for 20 mm and quadr.uplic...
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Nucleoside diphosphate (NDP) kinase from Escherichia coli was purified to homogeneity and was crystallized. Gel filtration analysis of the purified enzyme indicated that it forms a tetramer. The enzyme was phosphorylated with [gamma-32P]ATP, and the pH stability profile of the phosphoenzyme indicated that two different amino acid residues were phosphorylated. Both a histidine residue and serine...
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Soluble nucleoside-diphosphate kinase (NDP kinase) from Candida albicans was purified to electrophoretic homogeneity and a partial sequence was determined. The enzyme was kinetically and physically characterized. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme revealed a single polypeptide of 17 kDa upon staining, by immunodetection with heterologous and homolog...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biochemistry
سال: 2009
ISSN: 0300-8177,1573-4919
DOI: 10.1007/s11010-009-0123-4